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Structured Review

Merck KGaA pet-28(+) vector
Pet 28(+) Vector, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-28(+)+vector/pet28a/pmc10967249-46-1-25
Average 90 stars, based on 1 article reviews
pet-28(+) vector - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: CRISPR–Cas9-mediated genome editing in apple and grapevine
Article Snippet: The CRISPR–Cas9 genome-editing tool and the availability of whole-genome sequences from plant species have revolutionized our ability to introduce targeted mutations into important crop plants, both to explore genetic changes and to introduce new functionalities.. Here, we describe protocols adapting the CRISPR–Cas9 system to apple and grapevine plants, using both plasmid-mediated genome editing and the direct delivery of CRISPR–Cas9 ribonucleoproteins (RNPs) to achieve efficient DNA-free targeted mutations in apple and grapevine protoplasts.. We provide a stepwise protocol for the design and transfer of CRISPR–Cas9 components to apple and grapevine protoplasts, followed by verification of highly efficient targeted mutagenesis, and regeneration of plants following the plasmid-mediated delivery of components.

Expressing:

Article Title: CRISPR–Cas9-mediated genome editing in apple and grapevine
Article Snippet: The CRISPR–Cas9 genome-editing tool and the availability of whole-genome sequences from plant species have revolutionized our ability to introduce targeted mutations into important crop plants, both to explore genetic changes and to introduce new functionalities.. Here, we describe protocols adapting the CRISPR–Cas9 system to apple and grapevine plants, using both plasmid-mediated genome editing and the direct delivery of CRISPR–Cas9 ribonucleoproteins (RNPs) to achieve efficient DNA-free targeted mutations in apple and grapevine protoplasts.. We provide a stepwise protocol for the design and transfer of CRISPR–Cas9 components to apple and grapevine protoplasts, followed by verification of highly efficient targeted mutagenesis, and regeneration of plants following the plasmid-mediated delivery of components.

other:

Article Title: Unique Ganglioside Recognition Strategies for Clostridial Neurotoxins
Article Snippet: HCR Expression and Purification DNAs encoding CNT HCRs were subcloned into the pET-28 expression vector (Merck KGaA, Darmstadt, Germany).

Article Title: Identification of promoter targets by Aureochrome 1a in the diatom Phaeodactylum tricornutum
Article Snippet: Each pET-28(+) vector, harboring individual full-length PtAureo1a , PtAureo1b , PtAureo1c , PtAureo2 , and PtBlindA1a genes, was transformed into Escherichia coli Rosetta (DE3) cells (Merck Millipore, Darmstadt, Germany) for expression of the respective PtAUREO and PtBLINDA1a recombinant proteins tagged with six histidines (His6) at the N-termini.

Article Title: Glycosylated SV2 and Gangliosides as Dual Receptors for Botulinum Neurotoxin Serotype F
Article Snippet: DNA encoding HCR/A (residues 870-1295; Supplemental Figure 1 ) and HCR/F (residues 862-1278; Supplemental Figure 2 ) were subcloned into the pET-28 expression vector (Merck KGaA, Darmstadt, Germany).

Article Title: Identification of promoter targets by Aureochrome 1a in the diatom Phaeodactylum tricornutum.
Article Snippet: Expression and characterization of recombinant PtAUREO proteins Each pET-28(+) vector, harboring individual full-length PtAureo1a, PtAureo1b, PtAureo1c, PtAureo2, and PtBlindA1a genes, was transformed into Escherichia coli Rosetta (DE3) cells (Merck Millipore, Darmstadt, Germany) for expression of the respective PtAUREO and PtBLINDA1a recombinant proteins tagged with six histidines (His6) at the N-termini.

Article Title: Unique Ganglioside Recognition Strategies for Clostridial Neurotoxins
Article Snippet: DNAs encoding CNT HCRs were subcloned into the pET-28 expression vector (Merck KGaA, Darmstadt, Germany).



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